Why You Should Consider Hollow Fibres for Ultrafiltration

According to conventional wisdom within the biopharmaceutical industry, cassettes are the most suitable tangential flow filtration (TFF) technology for ultrafiltration applications such as protein or virus concentration and diafiltration. However, Parker domnick hunter is challenging this concept with the addition to its range of a new single-use TFF filter in the hollow fibre format.

Parker domnick hunter has extensive experience in the field of single-use TFF automation and has provided biomanufacturers with single-use manifolds to fit automated systems. Into these manifolds, customers have incorporated a reusable cassette at their facility. Recognizing that it was beneficial to supply completely disposable fluid flow-paths with an ultrafiltration filter incorporated, Parker domnick hunter has now launched PROPOR TFF single-use hollow fibre filters.


Why hollow fibre TFF technology?

Although hollow fibre filters are regularly found in microfiltration applications within bioprocessing, their use in ultrafiltration applications, where cassette formats dominate, has been limited. However, there are several benefits to using hollow fibre filter formats for ultrafiltration applications. These benefits were the drivers behind Parker domnick hunter’s decision to select the hollow fibre format for the range.

1. A hollow fibre format provides a more cost effective method for packaging filter membrane.

2. There is no requirement for cassette holders which reduces capital costs.

3. Hollow fibres allow completely pre-assembled, pre-sterilized, disposable flow-paths.

4. Closed system and sterile processing is enabled.


No pre-flush required

PROPOR TFF single-use hollow fibre filters contain a modified PES (Polyethersulphone) membrane with extremely low binding properties leading to less fouling, greater yields, higher flux rates and shorter processing times.

Significantly the membrane was developed so as to not require a preservative and hence requires no pre-flushing. Figure 2 shows a comparison of the pre-flush required to reduce a glycerin-stored TFF filter’s extractables to the baseline level of the PROPOR TFF single-use filter.

Reduce set up and tear down time

PROPOR TFF single-use hollow fibre filters can save over five hours of facility time by eliminating cassette installation, pre- and post-use water permeability testing, pre-flushing, pre- and post-use cleaning, and cassette storage operations (see Figure 3).

After being presented with these benefits during Parker domnick hunter’s recent webinar, Best Practices for Developing Single-Use TFF Steps, attendees were asked the question “Might single-use TFF hollow fibres be a viable option for ultrafiltration in your process?” An overwhelming 77% voted yes (n=86). 

What do you think? Do you agree with the webinar poll respondents? Let us know in the comments section below.  

Parker domnick hunter, specializes in automating and controlling single-use processes. By integrating sensory and automation technology into a process, a manufacturer can control the fluid flow more effectively, ensuring the quality of the final product. Find out more about Parker domnick hunter's single-use TFF solutions.



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Understanding and Controlling Bioprocess Variation

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